These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Membrane disintegration and hemolysis of human erythrocytes by snake venom cardiotoxin (a membrane-disruptive polypeptide).
    Author: Chen YH, Hu CT, Yang JT.
    Journal: Biochem Int; 1984 Feb; 8(2):329-38. PubMed ID: 6477605.
    Abstract:
    The action of 7.2 microM cardiotoxin on 0.25% (v/v) human erythrocytes in plasma extender solution was studied by osmotic fragility and hemolysis. The toxin loosened the membrane rigidity without lysis for about 25 min and then the cells hemolyzed for about 6 h at 37 degrees. The membrane was not perturbed at 15 degrees. The hemolysis of cells was optimal at 37 degrees and virtually stopped below 20 degrees. Both events could be suppressed by adding inorganic salts or toxin antibody. Once the membrane was loosened, the addition of phospholipase A2 would potentiate the disintegration of erythrocytes. In contrast, hemolysis of erythrocytes by (Lys)n lacked a non-lytic period and was temperature independent and insensitive towards salts and phospholipase A2.
    [Abstract] [Full Text] [Related] [New Search]