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  • Title: Analysis of asparagine-linked oligosaccharides from plasma membranes of rat normal liver and ascites hepatoma cells.
    Author: Takahashi N, Watanabe T, Kojima K, Ito M, Shimizu S.
    Journal: Biochem Int; 1984 May; 8(5):639-45. PubMed ID: 6477625.
    Abstract:
    Isolated plasma membranes from rat liver and ascites hepatoma cells were shown by SDS-polyacrylamide gel electrophoresis and concanavalin A reactivity to contain a variety of glycoproteins having asparagine-linked oligosaccharides. Membrane oligosaccharides were released by almond glycopeptidase digestion, and the pyridylamino derivatives were analyzed by high-performance liquid chromatography. Forty-four percent of the total carbohydrates in the original membranes were released and suggested to be of the complex type. Hepatoma membranes showed different oligosaccharide patterns from normal.
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