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  • Title: Purification and kinetic characterisation of human erythrocyte actin.
    Author: Tilley L, Ralston G.
    Journal: Biochim Biophys Acta; 1984 Oct 09; 790(1):46-52. PubMed ID: 6477938.
    Abstract:
    Human erythrocyte actin can be extracted from membrane ghosts by low ionic strength treatment in the presence of protective amounts of calcium and ATP. Purification then involves a single chromatographic step. The erythrocyte actin can be labelled with N-(1-prenyl)iodoacetamide. The fluorescence enhancement which accompanies polymerisation can be used to determine the critical concentration for assembly and to follow the polymerisation reaction time-course. The polymerisation kinetics of erythrocyte actin are compared with those of rabbit skeletal muscle actin. The two are shown to be markedly different.
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