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  • Title: Isolation of toxigenic strains of Pasteurella multocida from lungs of pneumonic swine.
    Author: Pijoan C, Lastra A, Ramirez C, Leman AD.
    Journal: J Am Vet Med Assoc; 1984 Sep 01; 185(5):522-3. PubMed ID: 6480465.
    Abstract:
    Lungs from 113 pneumonic pigs were examined for Pasteurella multocida. The lungs were smeared directly onto blood agar and homogenized in brain-heart infusion broth and then inoculated intraperitoneally in mice. Pasteurella multocida isolates were typed for serotypes A (by hyaluronidase inhibition of capsule) and D (by acriflavine autoagglutination). Strains were tested for toxin production by intradermal injection of 0.2 ml of filtered 24-hour culture supernatants into guinea pigs. Most lungs (70.8%) yielded isolations. Most isolants (87.5%) were type A and 12.5% were type D. Of the type D strains, 80% were toxigenic. Of the type A isolants, 18.2% were toxigenic.
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