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  • Title: Autoradiography of ganglioside antigens separated by high-performance thin-layer chromatography with their antibodies.
    Author: Kasai N, Naiki M, Yu RK.
    Journal: J Biochem; 1984 Jul; 96(1):261-4. PubMed ID: 6490603.
    Abstract:
    An improved method is described for the detection of ganglioside antigens with the antibodies on thin-layer chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chromatographic plate, application of antibody solution, and detection of bound antibody with [125I]staphylococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an anti-glycolipid antibody.
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