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  • Title: High pressure liquid chromatographic determination of strychnine, using a reverse phase solvent system.
    Author: Crouch MD, Short CR.
    Journal: J Assoc Off Anal Chem; 1978 May; 61(3):612-5. PubMed ID: 649554.
    Abstract:
    A simple, rapid extraction and subsequent determination for strychnine, using high pressure liquid chromatography (HPLC) with a reverse phase solvent system, is described. Stomach contents or grain bait containing strychnine were made alkaline with sodium hydroxide and extracted with chloroform. Extract filtrates were injected directly into a liquid chromatograph without further preparation except for dilution, if necessary. Peaks were resolved within 3.5 min and peak heights were used for quantitation. HPLC of strychnine was carried out on a 30 cm X 4 mm id stainless steel column packed with micronBondapak C18. The solvent program was 0.005M phosphate buffer-methanol (60+40) at a flow of 1.5 ml/min. Recovery from spiked stomach contents was 93.9 +/- 3.5%. The detection capability for strynchnine, using the 254 nm ultraviolet detector, was 5 ng. The strychnine peak was collected and subjected to thin layer chromatography with strychnine standards for confirmation.
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