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  • Title: A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation.
    Author: Naqui A, Chance B.
    Journal: Anal Biochem; 1984 Aug 15; 141(1):179-83. PubMed ID: 6496926.
    Abstract:
    A semi-integrated method for the determination of the enzyme kinetics parameters (Km and V) and graphical representation of the Michaelis-Menten equation is proposed as a variation of determination of initial reaction rate (v) as a function of initial substrate concentration ([S]0). The method is based on the determination of the time required to exhaust half of the initial substrate concentration as a function of the initial substrate concentration. The advantages and limitations of this method are discussed.
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