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Title: High-performance liquid chromatography of retinals, retinols (vitamin A1) and their dehydro homologues (vitamin A2): improvements in resolution and spectroscopic characterization of the stereoisomers. Author: Zonta F, Stancher B. Journal: J Chromatogr; 1984 Sep 28; 301(1):65-75. PubMed ID: 6501494. Abstract: A study of mobile phases for the improved high-performance liquid chromatographic resolution of retinal, 3-dehydroretinal, retinol and 3-dehydroretinol stereoisomers is described. By using 1-octanol as a phase modifier in n-hexane, the simultaneous separation of a complex mixture of vitamin A1 and A2 isomers was satisfactorily achieved, while the separation of the corresponding aldehydes required a ternary mixture of 2-propanol, dioxane and n-hexane. All peaks were spectroscopically characterized by recording their UV spectra, which are reported together with comparative tables of the absorbance maxima of the stereoisomers.[Abstract] [Full Text] [Related] [New Search]