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  • Title: A new method for preparation of isolated single adult myocytes.
    Author: Bkaily G, Sperelakis N, Doane J.
    Journal: Am J Physiol; 1984 Dec; 247(6 Pt 2):H1018-26. PubMed ID: 6507636.
    Abstract:
    A new technique for isolation of single cardiac cells from adult rat ventricles is described. The mechanical method is modified from the method of Jacobson (Cell Struct. Funct. 2: 1-9, 1977) by employing an enzyme-free dispersion medium containing Sr2+ or Ba2+ to replace Ca2+. This method produces a high yield of isolated rod-shaped adult mammalian cardiomyocytes that do not contract spontaneously, have a normal resting potential, and are Ca2+ tolerant. This method represents a promising technique for isolation of single, viable, intact, adult myocytes.
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