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  • Title: Competitive inhibition of human mitochondrial NADH dehydrogenase by Cibacron Blue F3GA.
    Author: Tarsio JF, Shapiro BL.
    Journal: Enzyme; 1984; 32(3):188-92. PubMed ID: 6510405.
    Abstract:
    Cibacron Blue F3GA, the chromophore of blue dextran, was tested at 4-16 mumol/l for possible inhibition of NADH dehydrogenase activity when added to mitochondrial preparations from cultured human skin fibroblasts. The free dye was shown to be a competitive inhibitor for NADH in the oxidation of NADH catalyzed by the mitochondrial enzyme. The Ki (5.8 mumol/l) for Cibacron Blue F3GA was considerably lower than the Michaelis constant (Km) found for NADH substrate (13.2-16.1 mumol/l), indicating a strong binding of the dye to the substrate-binding site of the enzyme. This is the first report of the competitive inhibition by Cibacron Blue F3GA of mitochondrial NADH in any species.
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