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  • Title: Ultraviolet irradiation mutagenesis and recombination in Spiroplasma citri.
    Author: Labarere J, Barroso G.
    Journal: Isr J Med Sci; 1984 Sep; 20(9):826-9. PubMed ID: 6511358.
    Abstract:
    A method for obtaining and screening uv-induced mutants from Spiroplasma citri is described. Lethality response curves showed that S. citri is more sensitive to uv irradiation than are other microorganisms. The presence of a shoulder in the lethality response curve showed the existence of systems able to repair uv-induced DNA damages. Toxic-resistant mutants have been obtained. A uv fluence equal to 10 J/m2 multiplied by 2.5 X 10(3) gave the spontaneous mutation frequency. Arsenic acid- and xylitol-resistant mutants were used to investigate transfer of genetic information in S. citri. After 90 min of incubation, the recombination frequency was 5 X 10(-5).
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