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  • Title: Separation of brain monosialoganglioside molecular species by high-performance liquid chromatography.
    Author: Kadowaki H, Evans JE, McCluer RH.
    Journal: J Lipid Res; 1984 Oct; 25(10):1132-9. PubMed ID: 6512419.
    Abstract:
    A method for the separation of molecular species of brain monosialogangliosides by high-performance liquid chromatography is described. GM4, GM3, GM2, and GM1 were purified from human brain and their individual molecular species were separated on a C18 reversed-phase column. Peaks were identified by mass spectrometry of the intact ganglioside, by gas-liquid chromatography of the fatty acids, and by high-performance liquid chromatography of the long chain bases. A characteristic elution sequence of molecular species permitted their identification based upon their retention times on the reversed-phase column.
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