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  • Title: [Subcellular distribution and properties of aldehyde dehydrogenase in the rat liver].
    Author: Konoplitskaia KL, Kuz'mina GI, Kuz'menko LA.
    Journal: Ukr Biokhim Zh (1978); 1984; 56(6):624-8. PubMed ID: 6515731.
    Abstract:
    The subcellular distribution and certain properties of rat liver aldehyde dehydrogenase are investigated. The enzyme is shown to be localized in fractions of mitochondria and microsomes. Optimal conditions are chosen for detecting the aldehyde dehydrogenase activity in the mentioned fractions. The enzyme of mitochondrial fraction shows the activity at low (0,03-0.05 mM; isoenzyme I) and high (5 mM; isoenzyme II) concentrations of the substrate. The seeming Km and V of aldehyde dehydrogenase from fractions of mitochondria and microsomes of rat liver are calculated, the acetaldehyde and NAD+ reaction being used as a substrate.
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