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  • Title: High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides.
    Author: Orr GA, Blanchard JS.
    Journal: Anal Biochem; 1984 Oct; 142(1):232-4. PubMed ID: 6517317.
    Abstract:
    High-performance anion-exchange chromatography of oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) on a Pharmacia Mono Q anion-exchange column is reported. Microgram quantities of all four nucleotides can be separated at pH 7.7 in approximately 20 min. For preparative purposes, greater than 7 mg of NADH can be purified in a single injection, and the peak fractions have an A260 of greater than 80 OD units with an A260/A340 ratio of 2.25.
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