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  • Title: Ultrastructural localization of lectin binding sites of the acrosomal membrane system of boar spermatozoa.
    Author: Töpfer-Petersen E, Januschke E, Schmoeckel C, Schill WB.
    Journal: Andrologia; 1984; 16(6):538-47. PubMed ID: 6517360.
    Abstract:
    The distribution of the main lectin receptors of boar spermatozoa were analyzed by concanavalin A and Ricinus communis agglutinin-120 visualized for light microscopy by enzymatic amplification with 3-amino-9-ethylcarbazole. The lectin binding sites of the acrosomal membranes in addition to the plasma membrane were displayed by electron microscopy using the lectin-peroxidase and lectin-ferritin technique. Lectin binding was particularly prominent at the anterior part of the head. It could be shown that both acrosomal membranes contain receptor sites for concanavalin A and Ricinus communis-agglutinin-120. Additionally, the whole cell surface could be labeled with both lectins.
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