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  • Title: Production of monoclonal antibodies to staphylococcal enterotoxin A.
    Author: Edwin C, Tatini SR, Strobel RS, Maheswaran SK.
    Journal: Appl Environ Microbiol; 1984 Dec; 48(6):1171-5. PubMed ID: 6517583.
    Abstract:
    Spleen cells from mice immunized with staphylococcal enterotoxin A were successfully fused with NS-1 mouse myeloma cells. Two of the four clones studied produced monoclonal antibodies to staphylococcal enterotoxin A in growth medium which showed titers of greater than 10(6) to 10(7) when tested by the indirect enzyme-linked immunosorbent assay. These monoclonal antibodies showed reactivity with enterotoxins A and E in the enzyme-linked immunosorbent assay. However, the reactivity was higher with enterotoxin A than with enterotoxin E. Nanogram quantities of crude staphylococcus enterotoxin A from Staphylococcus aureus growth were detected by the monoclonal antibodies in electroimmunoblots via autoradiography.
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