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  • Title: Effects of sulfhydryl reagents on uterine nuclear estrogen receptors labeled by in vitro exchange with [3H]estradiol or [3H]4-hydroxytamoxifen.
    Author: Attardi B.
    Journal: J Recept Res; 1984; 4(7):787-800. PubMed ID: 6527361.
    Abstract:
    We have attempted to convert 4 S uterine nuclear estrogen receptors obtained after in vitro labeling with [3H]antiestrogens to 3 S, the form observed after in vitro exchange with [3H]estradiol, in order to examine the possible relationship between these forms. Treatment of nuclear extracts labeled with the high affinity antiestrogen, [3H]4-hydroxytamoxifen, with a variety of nucleases, phosphatases, or proteases either had no effect on the 4 S antiestrogen-receptor complex or led to loss of ligand binding. The sulfhydryl reducing agents, cysteine or reduced glutathione, on the other hand, brought about conversion of 4 S estrogen receptors to components sedimenting at about 3 S. Conversely, when oxidized glutathione was included in all buffers used for preparation and labeling of nuclear estrogen receptors with [3H]estradiol, more rapidly sedimenting (approximately 4.6 S) forms of estrogen-receptor complex predominated. Cysteine still effected the 4 S to 3 S conversion when nuclear estrogen receptors, partially purified by sucrose gradient centrifugation, were used as substrate, suggesting a direct action of the sulfhydryl reagents on receptor molecules. From these results we propose that nuclear estrogen and antiestrogen-receptor complexes may differ in conformation such that the former may be more sensitive to the action of an endogenous reducing agent which contributes to formation of 3 S [3H]estradiol-receptor complexes.
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