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  • Title: Stabilization of ascorbic acid and norepinephrine in vitro by the subcellular fractions of rat cerebral cortex.
    Author: Kovachich GB, Mishra OP.
    Journal: Neurosci Lett; 1984 Nov 23; 52(1-2):153-8. PubMed ID: 6527832.
    Abstract:
    The effect of various subcellular fractions of rat cerebral cortex on the autoxidation of ascorbic acid and norepinephrine in a physiological salt solution, pH 7.4, was investigated. The rate of the two reactions was determined spectrophotometrically at 265 and 480 nm, respectively. The cytoplasmic, microsomal, crude mitochondrial fractions and a soluble phase of mixed subcellular origin (100,000 g supernatant) were tested in an assay system in which the final dilution of the cellular components was in the 10(3) to 10(6) range. The samples were heat-treated (30 min at 100 degrees C) and dialyzed (50,000 mol.wt. cut-off) prior to analysis. The preparations produced approximately 80% inhibition at 10(3)-fold dilution and marginal inhibition at 10(5)-fold dilution, except the microsomal preparation, which was a considerably weaker inhibitor in the norepinephrine autoxidation test. The study shows that all three major subcellular compartments of the rat cerebral cortex have macromolecules with a high level of heat-stable autoxidation-inhibiting activity.
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