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  • Title: A rapid preparative method for isolation of neutral and acidic glycosphingolipids by radial thin-layer chromatography.
    Author: Das KK, Basu M, Basu S.
    Journal: Anal Biochem; 1984 Nov 15; 143(1):125-34. PubMed ID: 6528990.
    Abstract:
    An efficient method to separate neutral and acidic glycosphingolipids (GSLs) from their mixtures within a short period (45-60 min) and with low consumption of solvents (chloroform-methanol-water, 60/35/8 (v/v/v); 250-500 ml) has been developed. This method utilizes a centrifugal thin-layer chromatograph (Chromatotron) and the GSL mixtures (30-400 mg) are applied to glass plates coated with a 1-mm layer of silica gel 60 PF-254. The method (radial thin-layer chromatography) is rapid and simple and the recovery of glycosphingolipids is high (70-80%).
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