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  • Title: [Normal values of the spontaneous cytochemical NBT test in adults].
    Author: Dragomirescu T, Bordeianu A.
    Journal: Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol; 1978; 23(1):43-6. PubMed ID: 653216.
    Abstract:
    The description is given of a working method adapted after Park, Fikrig and Smithwick, for testing the capacity of neutrophils to reduce tetrazolium nitroblue salts spontaneously. In contrast to the original technique, the present method uses : 10-20 units indigenous heparin/ml blood, leukocyte concentrate, other reaction times at 37 degrees C, contrast staining with a week Giemsa solution. Applied to young healthy subjects, the mean proportion of NBT-positive neutrophils was of 4.3 +/- 3.09 and the absolute number approximately less than 700/ml blood. The results obtained were consistent with the published data.
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