These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Quantitation of free and conjugated bile acids in human feces using a high-pressure liquid chromatography counterion method.
    Author: Ferguson LR, Rewcastle GW, Lello JM, Alley PG, Seelye RN.
    Journal: Anal Biochem; 1984 Dec; 143(2):325-32. PubMed ID: 6532247.
    Abstract:
    A method has been developed for extraction and purification of the major bile acids in human feces, and for their quantitative estimation using high-pressure liquid chromatography. Freeze-dried fecal material was extracted with alkaline ethanol and, after removal of neutral steroids, was subjected to thin-layer chromatography, followed by reversed-phase C18 silica cartridge (Sep-Pak) purification. The mixture was further separated into free, and glyco and tauro conjugates by ion-exchange chromatography. Subsequent resolution of individual bile acids was accomplished by HPLC using a counterion pairing method.
    [Abstract] [Full Text] [Related] [New Search]