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  • Title: Distribution of filipin-sterol complexes in rat hepatocytes.
    Author: Murakoshi M, Satoh S, Osamura Y, Watanabe K.
    Journal: Tokai J Exp Clin Med; 1984 Mar; 9(1):21-6. PubMed ID: 6535297.
    Abstract:
    Distribution of cholesterol in rat hepatocytes under normal untreated and phenobarbital-treated conditions was studied by freeze-fracturing using filipin. In untreated rat hepatocytes, filipin-sterol complexes were observed on intercellular plasma membranes as well as on lysosomes and microbodies. These complexes were evenly distributed. In phenobarbital-treated rat hepatocytes, filipin-sterol complexes in the lysosomes or microbodies were distributed unevenly. In the bile canaliculi, no filipin-sterol complexes were noted. Based on these findings, the functional state of the organellae concerned was discussed.
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