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  • Title: Synthesis of three N-nitroso dipeptides N-terminal in proline and a method for their determination in food.
    Author: Tricker AR, Perkins MJ, Massey RC, McWeeny DJ.
    Journal: Food Addit Contam; 1984; 1(4):307-12. PubMed ID: 6537354.
    Abstract:
    The terminal N-nitroso derivatives of L-prolyl-L-alanine, L-prolylglycine and L-prolyl-4-hydroxy-L-proline have been synthesized and characterized. A procedure suitable for their analysis in biological samples has been developed and is based on the preparation of an aqueous extract, extraction into an organic solvent, separation by high-performance liquid chromatography and detection by a chemiluminescence method. The average recovery in all cases was above 69% at the level of 200 micrograms/kg using cured meat as a food matrix; the detection limit was 10 micrograms/kg.
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