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  • Title: Evaluation of methods for the determination of Q and K antigens of an 02:K1(L) strain of Escherichia coli.
    Author: Deb JR, Harry EG.
    Journal: J Med Microbiol; 1977 Feb; 10(1):77-85. PubMed ID: 65474.
    Abstract:
    Tests made on ten colonies from a strain of Escherichia coli O2:K1 demonstrated that bacterial agglutination tests were reliable for identifying the O antigen of serogroup O2 but were unreliable for identifying the K1 antigen. The granular nature of K agglutination was not a reliable characteristic of the L type of K antigen. In contrast, indirect haemagglutination, immunodiffusion and immunoelectrophoresis tests with bacterial extracts gave consistent results with all colonies. The polysaccharide K1 antigen formed a long anodic precipitation line with two peaks, indicating its heterogenous nature, and partial fusion of this line with the O-antigen precipitation line suggested the presence of common serological determinants. In addition, a heat-labile protein antigen, possibly another K antigen, was identified by indirect haemagglutination tests and may have produced a short anodic precipitation line. The results also showed that the K1 antigen was still produced after storage of a culture for 12 years on Dorset-egg medium.
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