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  • Title: Molecular cloning of cDNA for rat liver catalase.
    Author: Osumi T, Ozasa H, Miyazawa S, Hashimoto T.
    Journal: Biochem Biophys Res Commun; 1984 Jul 31; 122(2):831-7. PubMed ID: 6547842.
    Abstract:
    For the studies on the induction of peroxisomal enzymes by hypolipidemic agents, we have tried to isolate a cDNA clone for rat liver catalase. A recombinant clone, pMJ501, was isolated, of which cDNA insert specifically hybridized to catalase mRNA in hybridization-selected translation. On RNA blot hybridization, it hybridized to 2.4-kilobases RNA which was increased about 1.5-fold by the administration of di-(2-ethylhexyl)phthalate to the rats. The nucleotide sequence of the cDNA contains a reading frame for 109 amino acid residues which match the reported amino acid sequence of bovine liver catalase at the carboxyl end with 82% homology. It is concluded that pMJ501 contains a cDNA sequence for rat liver catalase.
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