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  • Title: Calcium transport by rat brain mitochondria and oxidation of 2-oxoglutarate.
    Author: Bernard PA, Cockrell RS.
    Journal: Biochim Biophys Acta; 1984 Sep 27; 766(3):549-53. PubMed ID: 6548153.
    Abstract:
    Contrary to previous reports brain mitochondria have a substantial capacity for net Ca2+ uptake (approx. 1.2 mueq. Ca2+ per mg protein) providing succinate is the oxidizable substrate. ATP stimulates calcium uptake (to 1.8 mueq. per mg protein), but is not required. The accumulation of Ca2+ with NAD-linked substrates is, however, significantly less. With 2-oxoglutarate, very limited Ca2+ uptake occurs before respiration is inhibited. At low concentrations (10 microM), Ca2+ stimulates the 2-oxoglutarate dehydrogenase activity of detergent solubilized mitochondria. Millimolar [Ca2+] is required for inhibition. Therefore, Ca2+ inhibition of 2-oxoglutarate oxidation can explain the low maximum uptake with this substrate, but probably not by directly effecting the dehydrogenase. Hence, the oxidation of 2-oxoglutarate can be either enhanced or suppressed depending upon the net Ca2+ accumulated by brain mitochondria.
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