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  • Title: Orthovanadate induces loss of muscarinic cholinergic binding sites.
    Author: Danielsson E, Undén A, Bartfai T.
    Journal: Biochem Biophys Res Commun; 1983 Jan 27; 110(2):567-72. PubMed ID: 6551164.
    Abstract:
    In homogenates of corpus striatum from rats, incubated in the presence of adenosine triphosphate (2 mM) (45 min, 37 degrees C), a loss (approx. 50%) of muscarinic cholinergic binding sites could be detected using a 3H-3-Quinuclidinyl benzilate (3H-3-QNB) binding assay. We found that only vanadium-contaminated ATP induced loss of muscarinic binding sites. Orthovanadate (1 mM) alone in the absence of ATP induced loss (approx. 60%) of binding sites just as "vanadium-ATP" did.
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