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  • Title: Chromatographic method for the isolation of active 40S and 60S subunits from rat liver polyribosomes.
    Author: Kopacz-Jodczyk T, Paszkiewicz-Gadek A, Lopaczyński W, Gałasiński W.
    Journal: J Chromatogr; 1984 Apr 06; 287(2):329-35. PubMed ID: 6563039.
    Abstract:
    A rapid and simple procedure for isolation of 40S and 60S ribosomal subunits by ion-exchange column chromatography is described. The dissociated ribosomes can be separated and non-ribosomal proteins and low-molecular-weight substances removed. An assessment by physicochemical and functional criteria showed that the ribosomal subunits obtained are active and sufficiently homogeneous.
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