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  • Title: In vitro prothrombin synthesis from a purified precursor protein. II. Partial purification of bovine carboxylase.
    Author: Vermeer C, Soute BA, Hemker HC.
    Journal: Biochim Biophys Acta; 1978 Apr 12; 523(2):494-505. PubMed ID: 656438.
    Abstract:
    In this paper, we describe the isolation and partial purification of an enzyme system that converts bovine decarboxyfactor II (PIVKA-II) into prothrombin (factor II). It is shown that the increase in factor II activity occurs in parallel with 14CO2 incorporation into BaSO4 adsorbable proteins. The system is not strictly vitamin K-dependent because it is obtained from the livers of normal healthy cows. By preincubating the enzyme(s) with an excess of warfarin, an absolute vitamin K1-dependence can be obtained. The reaction is inhibited by its own product, factor II.
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