These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme.
    Author: Nishio K, Kawakami M.
    Journal: J Biochem; 1984 Dec; 96(6):1867-74. PubMed ID: 6570482.
    Abstract:
    Alanyl-tRNA synthetase was purified from the posterior silk glands of Bombyx mori by ammonium sulfate fractionation and chromatography on DEAE-Sephacel and hydroxyapatite columns. The yield was about 100 mg of the enzyme per 1 kg of the glands. The enzyme required both L-alanine and alanine tRNA for pyrophosphate formation from ATP. The PPi formation was observed even after tRNA was fully aminoacylated. The enzyme was found to be a monomer of 115K daltons by SDS-polyacrylamide gel electrophoresis, gel filtration and suberimidate cross-linking experiments. The monomeric enzyme did not dimerize in the presence of the alanine tRNA. The enzyme and the tRNA formed a 1:1 complex. The results indicate that Bombyx mori alanyl-tRNA synthetase functions in a monomeric state.
    [Abstract] [Full Text] [Related] [New Search]