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  • Title: Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry.
    Author: Smith BJ, Herold DA, Ross RM, Marquis FG, Bertholf RL, Ayers CR, Wills MR, Savory J.
    Journal: Res Commun Chem Pathol Pharmacol; 1983 Apr; 40(1):73-86. PubMed ID: 6575411.
    Abstract:
    A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethyl-silyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 +/- 2.0 pg/ml.
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