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  • Title: Relation between pH and ionized calcium in vitro and in vivo in man.
    Author: Thode J, Fogh-Andersen N, Wimberley PD, Møller Sørensen A, Siggaard-Andersen O.
    Journal: Scand J Clin Lab Invest Suppl; 1983; 165():79-82. PubMed ID: 6578581.
    Abstract:
    We investigated the in vitro and in vivo relation between pH and ionized calcium during acute respiratory pH changes. The in vitro slope delta 1gcCa2+/delta pH determined in 20 healthy volunteers after equilibration at two different pCO2 values was -0.22 +/- 0.04 (mean +/- 2 SD) for whole blood and -0.24 +/- 0.04 (mean +/- SD) for serum. The in vivo slope delta 1gcCa2+/delta pH during acute respiratory changes in 10 healthy volunteers was -0.17 +/- 0.04 (mean +/- 2 SD) for capillary blood. A mathematical formula which describes the relation between pH and ionized calcium is presented.
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