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  • Title: Interaction of the 2,6-dimethoxysemiquinone and ascorbyl free radicals with Ehrlich ascites cells: a probe of cell-surface charge.
    Author: Pethig R, Gascoyne PR, McLaughlin JA, Szent-Györgyi A.
    Journal: Proc Natl Acad Sci U S A; 1984 Apr; 81(7):2088-91. PubMed ID: 6585788.
    Abstract:
    The rate of quenching by Ehrlich ascites cells of anionic 2,6-dimethoxy-p-semiquinone and ascorbyl free radicals is investigated as a function of cell concentration, the blocking of cell-surface sulfhydryl groups by N-ethylmaleimide, and the reduction of cell-surface charge by neuraminidase. The rate of quenching is found to be proportional to cell viability and to the number of free cell-surface sulfhydryl groups. The enzymatic action of neuraminidase results in an increase of the free radical quenching rate, indicating that this rate can be used as a probe of cell-surface charge. Measurements as a function of the ionic strength of the suspending electrolyte gave a value of -1.22 microC X cm-2 for the charge density at the ascites cell surface. This is equivalent to a surface membrane potential of -14 mV for a 150 mM NaCl electrolyte and is a value in good agreement with published electrophoresis data.
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