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  • Title: Transfer from Rhizobium japonicum to Azotobacter vinelandii of genes required for nodulation.
    Author: Maier RJ, Bishop PE, Brill WJ.
    Journal: J Bacteriol; 1978 Jun; 134(3):1199-201. PubMed ID: 659367.
    Abstract:
    A mutant strain of Azotobacter vinelandii that is unable to fix N2 (Nif-) was transformed to Nif+ with DNA from Rhizobium japonicum. Of 50 Nif+ transformants tested, 3 contained the O antigen-related polysaccharide that is present on the cell surface of a nodulating R. japonicum strain, but is absent from a non-nodulating mutant strain.
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