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  • Title: Formation of 20-hydroxyprostaglandins by lungs of pregnant rabbits.
    Author: Powell WS, Solomon S.
    Journal: J Biol Chem; 1978 Jul 10; 253(13):4609-16. PubMed ID: 659437.
    Abstract:
    Homogenates or particulate fractions (1,000 to 100,000 X g) from lungs of pregnant rabbits were incubated with prostaglandins or prostaglandin metabolites and the products were purified by chromatography and identified by gas chromatography-mass spectrometry. In the presence of NADPH, particulate fractions from pregnant rabbit lungs converted prostaglandins E1, E2, and F2alpha as well as 13,14-dihydro-15-oxoprostaglandin E2 and 13, 14-dihydro-15-oxoprostaglandin F2alpha to their 20-hydroxy derivatives. In the cases of the 3 primary prostaglandins, the corresponding omega-carboxylic acids were also isolated. The omega-hydroxylation reaction occurred in the presence of the microsomal fraction. The mitochondrial fraction was much less active whereas the cytosol fraction converted prostaglandins to their 13, 14-dihydro-15-oxo derivatives. When prostaglandin F2alpha was incubated with homogenates of lungs from pregnant rabbits, omega-oxidation was combined with oxidation of the 15-hydroxyl group and reduction of the 13, 14-double bond to give 13, 14-dihydro-20-hydroxy-15-oxoprostaglandin F2alpha as well as the corresponding derivative with an omega-carboxylic acid group. Lungs from nonpregnant rabbits were much less active than lungs from pregnant rabbits in the omega-oxidation of prostaglandins.
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