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  • Title: Classification of newly formed and migrating splenic lymphocytes.
    Author: Pabst R, Licence ST, Binns RM.
    Journal: Clin Exp Immunol; 1983 Feb; 51(2):333-7. PubMed ID: 6601553.
    Abstract:
    Newly formed lymphoid cells in the spleens of normal young pigs were selectively labelled with 3H-TdR, using an extracorporeal perfusion system. One day later splenic and blood lymphocytes were separated by rosetting with sheep red blood cells for classical T cells and by a direct anti-globulin rosetting reaction for B cells. The proportion of newly formed lymphocytes in these subsets was determined by autoradiography. In this study we show that while both B and T lymphocytes are produced, B cells show a much higher incidence of newly formed cells. Labelled populations of spleen derived T and B lymphocytes were also found in the blood, but the B cells showed a lower labelling incidence than in the spleen.
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