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  • Title: Presence of immunoreactive corticotropin-releasing factor in human cerebrospinal fluid.
    Author: Suda T, Tozawa F, Mouri T, Demura H, Shizume K.
    Journal: J Clin Endocrinol Metab; 1983 Jul; 57(1):225-6. PubMed ID: 6602142.
    Abstract:
    Immunoreactive corticotropin-releasing factor (I-CRF) was measured by radioimmunoassay and immunoaffinity chromatography in human hypothalamus and cerebrospinal fluid (CSF). Dilution curves of I-CRF in the hypothalamus and CSF were parallel to that of synthetic ovine CRF standard. I-CRF content in the hypothalamus was 643 and 281 fmol eq, respectively. I-CRF concentration in CSF was 7.4 +/- 1.1 fmol eq/ml. Sephadex G-75 column chromatography showed the main peak eluted at the position of synthetic CRF.
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