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  • Title: Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence.
    Author: Hume DA, Gordon S.
    Journal: J Cell Physiol; 1983 Nov; 117(2):189-94. PubMed ID: 6605349.
    Abstract:
    A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.
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