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  • Title: Effect of T cell-derived lymphokines containing B cell differentiation factor(s) for IgG (BCDF gamma) on gamma-specific mRNA in murine B cells.
    Author: Jones S, Chen YW, Isakson P, Layton J, Pure E, Word C, Krammer PH, Tucker P, Vitetta ES.
    Journal: J Immunol; 1983 Dec; 131(6):3049-51. PubMed ID: 6605997.
    Abstract:
    Cytoplasmic RNA was isolated from cells cultured with LPS and LPS plus a T cell-derived supernatant (SN) (PK 7.1) containing B cell differentiation factors. The steady state levels of isotype-specific mRNA were assessed by Northern blot analysis with gamma-specific CH3 probes. It was demonstrated that the SN induces an increase in the level of mRNA for gamma 1 and a concomitant decrease in the levels of mRNA for gamma 2b and gamma 3.
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