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  • Title: Growth of ovine granulocyte-macrophage precursors in vitro without exogenous colony-stimulating activity.
    Author: Chandra P, Joel DD, Chanana AD.
    Journal: Am J Vet Res; 1983 Nov; 44(11):2070-2. PubMed ID: 6606371.
    Abstract:
    Ovine granulocyte-macrophage colony-forming units (CFU-GM) from peripheral blood and bone marrow were cultured in vitro. The colony-stimulating activity (CSA) was provided by various conditioned-media previously reported to contain CSA and by homologous sheep serum (SS). The maximum number of CFU-GM was observed in the cultures containing SS without the addition of exogenous CSA. The CFU-GM appeared earlier in the cultures containing bone marrow cells when compared to the peripheral blood CFU-GM. Replacement of SS by bovine fetal serum resulted in suboptimal growth of ovine CFU-GM.
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