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  • Title: Rapid diagnosis of congenital adrenal hyperplasia by high performance liquid chromatography.
    Author: Weisman Y, Bar A, Root A, Spirer Z, Golander A.
    Journal: Clin Chim Acta; 1984 Mar 27; 138(1):1-8. PubMed ID: 6609026.
    Abstract:
    This manuscript describes methods for the quantitation of serum concentrations of 17-hydroxyprogesterone and 11-deoxycortisol (Compound S) employing reverse-phase high-performance liquid chromatography (HPLC) and ultraviolet (UV) absorbance which are applicable to the diagnosis of congenital adrenal hyperplasia due to deficiencies of 21- and 11-hydroxylase activities, respectively. These methods are simple, specific, precise and rapid. Data obtained by the HPLC-UV methods are highly correlated (p less than 0.001) with radioimmunoassay measurements.
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