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  • Title: Depletion of murine anti-azobenzenearsonate plaque-forming cells by derivatized polyacrylamide beads.
    Author: Derbalian GP, Bellone CJ.
    Journal: Scand J Immunol; 1984 Apr; 19(4):343-51. PubMed ID: 6610207.
    Abstract:
    The depletion of secondary p-azobenzenearsonate plaque-forming cells (ABA-PFC) by affinity columns substituted with ABA was dependent on the primary dose, times elapsed after priming, and the nature of the side-arm on the columns. Thus, higher priming doses of ABA coupled to keyhole limpet haemocyanin (50-500 micrograms of ABA-KLH in complete Freund's adjuvant (CFA] favoured depletion of ABA-PFC by ABA coupled to a 6- aminocaproyltyrosine side-arm (SAC-TYR-ABA beads), whereas ABA-PFC from mice primed with 1 microgram of ABA-KLH in CFA were never depleted; this latter population were only depleted by ABA coupled to a histamine side-arm (HIST-ABA beads) 7 months after priming. These data are consistent with the notion that two specificities, HIST-ABA and TYR-ABA, appear to emerge independently, lower priming doses inducing the preferential appearance of the HIST-ABA specificity.
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