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  • Title: A detailed serological study of five anti-Jka sera reacting by the antiglobulin technique.
    Author: Howell P, Giles CM.
    Journal: Vox Sang; 1983; 45(2):129-38. PubMed ID: 6613080.
    Abstract:
    Detailed serological investigation of five examples of anti-Jka revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of complement, while two were detectable with anti-IgG, providing a spin test was used. Complement was bound by all and resulted in enhanced reactions, particularly when slide/tile tests were used. The study emphasizes that anti-complement (C3) is an essential component of polyspecific antiglobulin reagents used in cross-matching, enabling the ready detection of these clinically important antibodies.
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