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  • Title: Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins.
    Author: Beinert H.
    Journal: Anal Biochem; 1983 Jun; 131(2):373-8. PubMed ID: 6614472.
    Abstract:
    Details are provided for a reproducible procedure for determination of labile sulfide in iron-sulfur (Fe-S) proteins in the range of 1 to 3 nmol. Analyses are also presented on the most stable Fe-S protein so far reported. In this case denaturation with guanidine.HCl was used in the presence of dithiothreitol. The values obtained then also include any sulfane sulfur (S0) present.
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