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  • Title: Extracellular antigens of Micropolyspora faeni grown in synthetic medium.
    Author: Kurup VP, Fink JN.
    Journal: Infect Immun; 1977 Feb; 15(2):608-13. PubMed ID: 66202.
    Abstract:
    Extracellular antigens were prepared by growing Micropolyspora faeni in a new synthetic medium (SMSL), and this antigenic preparation was compared with antigens obtained by the double-dialysis method. These two preparations were tested against pooled sera from farmer's lung patients and rabbits immunized with these antigens by the agar-gel double-diffusion, immunoelectrophoresis, and two-dimensional immunoelectrophoresis methods. The immunological cross-reactivity between M. faeni strains of diverse origin and between other species of thermophilic actinomycetes associated with hypersensitivity pneumonitis was also investigated. The usefulness of two-dimensional immunoelectrophoresis in the comparison and standardization of antigens is discussed.
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