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  • Title: [Infectiousness of phage T4 frozen to -196 degrees in the presence of cryoprotectors].
    Author: Tsutsaeva AA, Vysekantsev IP.
    Journal: Mikrobiologiia; 1983; 52(3):519-21. PubMed ID: 6621427.
    Abstract:
    Polyethylene oxide with a molecular weight of 400 at a concentration of 5 to 15% and dimethyl sulfoxide at a concentration of 5% produce the protecting action when T4 phage is being frozen. The survival rate of the phage is lower when dimethyl sulfoxide at a concentration of 15% and glycerol and sucrose at a concentration from 5 to 15% are added to samples to be frozen as compared with samples frozen in the absence of the cryoprotectors.
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