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  • Title: Measurement of 3-methoxy-4-hydroxyphenylglycol in human plasma with high-performance liquid chromatography using electrochemical detection.
    Author: Scheinin M, Chang WH, Jimerson DC, Linnoila M.
    Journal: Anal Biochem; 1983 Jul 01; 132(1):165-70. PubMed ID: 6625157.
    Abstract:
    A new method utilizing high-performance liquid chromatography with electrochemical detection for the determination of unconjugated 3-methoxy-4-hydroxyphenylglycol, a major metabolite of norepinephrine, in human plasma is described. A novel internal standard, 3-ethoxy-4-hydroxyphenylglycol, was used to correct for variations in extraction efficiency and detector sensitivity. Separation of sample components was achieved isocratically in 20 min per sample in a reversed-phase system. Column temperature had to be carefully regulated. The sensitivity of the assay is suitable for clinical studies, with a limit of detection of 2 pmol/ml. Intra- and interassay precision was good, with observed coefficients of variation of 7.5 and 8.8%, respectively. Accuracy was tested by comparing the results of the present HPLC method to those found with a specific gas chromatographic-mass spectrometric assay. A satisfactory correlation of 0.96 was obtained, with no significant bias between the two methods.
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