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  • Title: Purification of reduced nicotinamide adenine dinucleotide by ion-exchange and high-performance liquid chromatography.
    Author: Newton CJ, Faynor SM, Northrop DB.
    Journal: Anal Biochem; 1983 Jul 01; 132(1):50-3. PubMed ID: 6625161.
    Abstract:
    Combining ion-exchange (AG MP-1) and reversed-phase (C-18) partition chromatography accomplishes a higher degree of purification of NADH than either method can provide alone. Final elution in 95% ethanol, dehydration with anhydrous sodium sulfate, and storage in dry 1,2-propanediol over molecular sieves prevents hydrolysis of the purified dinucleotide.
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