These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Separation and analysis of 4'-epimeric UDP-sugars by borate high-performance liquid chromatography.
    Author: Weckbecker G, Keppler DO.
    Journal: Anal Biochem; 1983 Jul 15; 132(2):405-12. PubMed ID: 6625174.
    Abstract:
    Separation of UDP-glucose from UDP-galactose, of UDP-N-acetylglucosamine from UDP-N-acetylgalactosamine, of UDP-glucuronate from UDP-galacturonate, or of UDP-glucosamine from UDP-galactosamine was achieved within 10-45 min by isocratic anion-exchange high-performance liquid chromatography (HPLC) using a flow rate of 2 ml/min. The eluants were composed of borate as complex-forming and eluting agent and of glycerol for protection of the alkali-labile silica packing of the column. This borate HPLC was suitable for the analysis of 4'-epimeric UDP-sugars in the range of 2 to 100 nmol. The applicability of this technique was demonstrated by determination of the relative amounts of 4'-epimeric UDP-amino sugars formed in rat liver after administration of D-galactosamine. Since a high salt content of UDP-sugar samples can interfere with borate HPLC, desalting was performed on a 1-ml C18 cartridge using triethylammonium hydrogen carbonate buffer. This procedure enabled the complete separation of various nucleotides from salts within 10 min prior to HPLC.
    [Abstract] [Full Text] [Related] [New Search]