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Title: Fitting enzyme-kinetic data to V/K. Author: Northrop DB. Journal: Anal Biochem; 1983 Jul 15; 132(2):457-61. PubMed ID: 6625178. Abstract: Kinetic data from enzyme-catalyzed reactions have been analyzed traditionally in terms of the Michaelis-Menten equation, which assumes that the maximal velocity (V) and the Michaelis constant (K) are the primary kinetic constants. But what is needed from most kinetic studies today is V/K. A new form of the equation is proposed which assumes that V and V/K are the primary kinetic constants: v = (V . S . V/K)/(V + S . V/K). Computer fittings of both experimental and simulated velocity data to both equations give results favoring the new equation.[Abstract] [Full Text] [Related] [New Search]